May 28, 2014: Stephanie Gupton, Department of Cell Biology & Physiology, UNC

Posted on May 19, 2014 by johannes

Our speaker this month is Dr. Stephanie Gupton, Assistant Professor in the Department of Cell Biology & Physiology, UNC Chapel Hill:

TRIM9 coordinates cytoskeletal dynamics and vesicle trafficking in developing neurons

3503 Thomas Hall (Stephens Room) 

Wednesday, May 28, 4pm (Refreshments at 3:30pm)

 

“We have recently identified the E3 ubiquitin ligase as a critical catalytic component downstream of the axon guidance cue Netrin during neuronal development. TRIM9 interacts with the Netrin receptor DCC, regulators of the actin cytoskeleton, and vesicle trafficking machinery.  Using biochemical techniques, high resolution microscopy, and mouse models we show that TRIM9 controls vesicle exocytosis, filopodia formation and axon guidance and branching.”

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April 23, 2014: Keith Weninger, Department of Physics, NCSU

Posted on April 18, 2014 by johannes

Our speaker this month is Dr. Keith Weninger, Associate Professor and University Faculty Scholar in the Department of Physics at NC State:

Connecting protein conformational dynamics with function using single molecule fluorescence microscopy

Auditorium DH Hill Library (2304 D.H. Hill Library) Wednesday, April 23, 4pm

Dr. Weninger developed the use of single molecule fluorescence resonance energy transfer to characterize the conformational dynamics of multimeric protein complexes:

“Observing single molecules allows discovery of properties that are typically hidden by averaging over the large number of unsynchronized molecules typical of biological samples. Single molecule fluorescence resonance energy transfer is a method that allows real-time observation of protein and DNA conformational changes with nanometer resolution. I will describe our applications of single molecule fluorescence microscopy to studies of proteins involved in several phenomena including DNA mismatch repair, neurotransmitter release and cancer related processes.”

 

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